Description
Albumin is a water-soluble protein with considerable structural stability which makes up 60 percent of the total protein of plasma. It functions as a carrier of hormones, enzymes, fatty acids, metal ions, and medicinal products. The sensitive quantitative measurement of total human albumin (human serum albumin, HSA) in plasma, serum, urine or other biological fluid samples is easily performed with this 96 well strip format ELISA kit. Albumin is present in normal human plasma at a concentration of 35-50 mg/ml. The assay measures human albumin in the 1-500 ng/ml range. Samples giving human albumin levels above 500ng/ml should be diluted in 1X diluent before use. A 1:1,000,000 to 1:2,000,000 dilution for normal plasma or a 1:500 to 1:1,000 dilution for urine is suggested for best results. Human albumin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, peroxidase labeled anti-human albumin primary antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of human albumin in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified human albumin and is measured along with the test samples. All reagents and standards are provided in these ELISA kits